Terry and Cole have been in a race against time to recover from ankle injuries for the west London derby at Loftus Road.
But they have trained without incident for the past three days and are set for a reunion with Anton Ferdinand, who Terry was cleared in court of of racially abusing back in July.
Cole gave evidence as a character witness for his team-mate during the court case and Terry, who was stripped of the England armband by the Football Association before his trial, is still facing a separate FA charge.
"They've recovered, had a couple of days training and we expect them to be available for tomorrow," Di Matteo said.
"We're going to respect the rules and the protocol that is in place from the Premier League and set a positive standard to the millions of people watching across the world."
Reports in the build-up to the West London derby claim
The handshake has been cancelled ahead of the clubs' last two meetings but Di Matteo confirmed: "We'll go ahead with it, yes."